5FBA
S1 nuclease from Aspergillus oryzae in complex with phosphate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.1 |
Synchrotron site | BESSY |
Beamline | 14.1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-05-30 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.91842 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 41.765, 62.307, 48.003 |
Unit cell angles | 90.00, 106.75, 90.00 |
Refinement procedure
Resolution | 45.970 - 1.800 |
R-factor | 0.15304 |
Rwork | 0.152 |
R-free | 0.19864 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ak0 |
RMSD bond length | 0.016 |
RMSD bond angle | 1.688 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0131) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.970 | 1.840 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.074 | 0.386 |
Number of reflections | 20922 | |
<I/σ(I)> | 11.7 | 2.2 |
Completeness [%] | 95.3 | 69 |
Redundancy | 3.1 | 2.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 291 | 0.2 M Sodium chloride, 0.1 M BIS-TRIS pH 5.5, 25% w/v Polyethylene glycol 3,350 |