5FBA
S1 nuclease from Aspergillus oryzae in complex with phosphate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-05-30 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.91842 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 41.765, 62.307, 48.003 |
| Unit cell angles | 90.00, 106.75, 90.00 |
Refinement procedure
| Resolution | 45.970 - 1.800 |
| R-factor | 0.15304 |
| Rwork | 0.152 |
| R-free | 0.19864 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ak0 |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.688 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0131) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.970 | 1.840 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.074 | 0.386 |
| Number of reflections | 20922 | |
| <I/σ(I)> | 11.7 | 2.2 |
| Completeness [%] | 95.3 | 69 |
| Redundancy | 3.1 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 291 | 0.2 M Sodium chloride, 0.1 M BIS-TRIS pH 5.5, 25% w/v Polyethylene glycol 3,350 |
