5FB6
Room-temperature macromolecular crystallography using a micro-patterned silicon chip with minimal background scattering
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 298 |
| Detector technology | PIXEL |
| Collection date | 2015-03-12 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.003 |
| Spacegroup name | I 21 3 |
| Unit cell lengths | 78.820, 78.820, 78.820 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 27.867 - 1.901 |
| R-factor | 0.1587 |
| Rwork | 0.157 |
| R-free | 0.17350 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 9ins |
| RMSD bond length | 0.009 |
| RMSD bond angle | 0.933 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.058 | 0.372 |
| Number of reflections | 6542 | |
| <I/σ(I)> | 18.04 | 5.16 |
| Completeness [%] | 99.2 | 99.69 |
| Redundancy | 5.2 | 5.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 0.6 M Na2HPO4 0.01 M EDTA |
