5FB4
Crystal structure of the bacteriophage phi29 tail knob protein gp9 truncation variant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 160 |
| Detector technology | CCD |
| Collection date | 2014-10-03 |
| Detector | RAYONIX MX-225 |
| Wavelength(s) | 0.978 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 94.600, 135.161, 313.431 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.317 - 2.039 |
| R-factor | 0.1547 |
| Rwork | 0.153 |
| R-free | 0.18320 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5fei |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.053 |
| Data scaling software | HKL-2000 |
| Phasing software | SHARP |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.320 | |
| High resolution limit [Å] | 2.039 | |
| Number of reflections | 126814 | |
| <I/σ(I)> | 17.66 | 9.2 |
| Completeness [%] | 99.3 | 95.11 |
| Redundancy | 6.1 | 5.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293.15 | 0.94% v/v ethanol, 10% PEG-400, HEPES buffer pH 7.5, magnesium chloride |
