5FB3

Structure of glycerophosphate dehydrogenase in complex with NADPH

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE AR-NW12A
Synchrotron site	Photon Factory
Beamline	AR-NW12A
Temperature [K]	100
Detector technology	CCD
Collection date	2015-06-12
Detector	ADSC QUANTUM 270
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	102.182, 123.335, 166.724
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 2.450
R-factor	0.2098
Rwork	0.207
R-free	0.25990
Structure solution method	SIRAS
RMSD bond length	0.008
RMSD bond angle	1.265
Data scaling software	HKL-2000
Refinement software	REFMAC (5.8.0073)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.490
High resolution limit [Å]	2.450	6.650	2.450
Rmerge	0.070	0.032	0.676
Rmeas	0.076	0.035	0.736
Rpim	0.029	0.014	0.288
Total number of observations	520646
Number of reflections	78117
<I/σ(I)>	7.2
Completeness [%]	100.0	99.5	100
Redundancy	6.7	6.5	6.5
CC(1/2)		0.998	0.898

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	ammonium sulfate