5FB2
S. aureus MepR F27L Mutant bound to oligodeoxyribonucleotide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E+ SUPERBRIGHT |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2014-04-12 |
| Detector | RIGAKU RAXIS HTC |
| Wavelength(s) | 1.54 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 32.263, 97.986, 106.317 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 36.026 - 1.800 |
| R-factor | 0.1921 |
| Rwork | 0.188 |
| R-free | 0.23180 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3eco |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.945 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 36.030 |
| High resolution limit [Å] | 1.800 |
| Rmerge | 0.044 |
| Number of reflections | 15799 |
| <I/σ(I)> | 10.5 |
| Completeness [%] | 98.1 |
| Redundancy | 2.81 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.2 | 298 | 20% (w/v) Peg 1000, 100 mM Potassium phosphate monobasic/Sodium phosphate dibasic buffer, 200 mM Sodium chloride |
