5FAD

SAH complex with aKMT from the hyperthermophilic archaeon Sulfolobus islandicus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	93
Detector technology	CCD
Collection date	2013-12-26
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9792
Spacegroup name	P 1 21 1
Unit cell lengths	38.162, 51.755, 40.596
Unit cell angles	90.00, 117.84, 90.00

Refinement procedure

Resolution	35.898 - 1.870
R-factor	0.1883
Rwork	0.183
R-free	0.21690
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.192
Refinement software	PHENIX (1.8.1_1168)

Data quality characteristics

	Overall
Low resolution limit [Å]	35.900
High resolution limit [Å]	1.870
Number of reflections	11289
<I/σ(I)>	33.35
Completeness [%]	93.7
Redundancy	3.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289	10M nickel chloride, 0.1M Tris-HCl, pH 8.5, 20%(w/v) PEG 2,000 MME