5FAA
Crystal structure of C-terminal domain of shaft pilin spaA from Lactobacillus rhamnosus GG, - I422 space group
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM14 |
Synchrotron site | ESRF |
Beamline | BM14 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-07-21 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.97625 |
Spacegroup name | I 4 2 2 |
Unit cell lengths | 100.240, 100.240, 57.539 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 28.890 - 1.600 |
R-factor | 0.1311 |
Rwork | 0.130 |
R-free | 0.15769 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3phs |
RMSD bond length | 0.008 |
RMSD bond angle | 1.221 |
Data scaling software | Aimless |
Phasing software | MR-Rosetta |
Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 28.890 | 1.750 |
High resolution limit [Å] | 1.600 | 1.600 |
Rmerge | 0.071 | 0.604 |
Number of reflections | 19599 | |
<I/σ(I)> | 37 | 6.4 |
Completeness [%] | 99.9 | 99.7 |
Redundancy | 28.5 | 29.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 295 | 0.1M sodium acetate, 25% PEG400 |