5F9U

X-RAY STRUCTURE OF THE ADDUCT BETWEEN HEN EGG WHITE LYSOZYME AND CISPLATIN UPON 24 HOURS OF INCUBATION AT 20 DEGREES

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	CCD
Collection date	2015-10-20
Detector	RIGAKU SATURN 944
Wavelength(s)	1.5418
Spacegroup name	P 43 21 2
Unit cell lengths	78.785, 78.785, 36.975
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	55.710 - 1.850
R-factor	0.17326
Rwork	0.170
R-free	0.23310
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	193l
RMSD bond length	0.018
RMSD bond angle	1.803
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	REFMAC
Refinement software	REFMAC (5.8.0135)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	55.710	1.880
High resolution limit [Å]	1.850	1.850
Rmerge	0.123	0.989
Number of reflections	10302
<I/σ(I)>	16.5	5.3
Completeness [%]	98.6	87.7
Redundancy	5.5	2.9
CC(1/2)		0.281

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	Crystals were grown using a reservoir solution of 1.1 M NaCl, 0.1 M sodium acetate pH 4.4 and a drop of 1 uL adduct solution and 1 uL reservoir solution.