5F9Q
Crystal structure of the extracellular domain of noncanonic ABC-type transporter YknZ from Gram-positive bacteria
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 173 |
Detector technology | CCD |
Collection date | 2015-11-08 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9797 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 44.169, 46.147, 87.543 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.823 - 2.044 |
R-factor | 0.1996 |
Rwork | 0.194 |
R-free | 0.24730 |
Structure solution method | SAD |
RMSD bond length | 0.010 |
RMSD bond angle | 1.157 |
Data scaling software | HKL-2000 |
Phasing software | AutoSol |
Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 41.000 | 10.000 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.093 | |
Number of reflections | 11741 | |
<I/σ(I)> | 12.6 | 12.6 |
Completeness [%] | 95.8 | 95.8 |
Redundancy | 4.7 | 4.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | EVAPORATION | 4.6 | 288 | 22.5% (w/v) polyethylene glycol 4000, 0.125 M ammonium acetate, 0.1 M sodium acetate trihydrate (pH 4.6) |