5F9C
Crystal structure of the G121R mutant of human phosphoglucomutase 1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 4.2.2 |
| Synchrotron site | ALS |
| Beamline | 4.2.2 |
| Temperature [K] | 100 |
| Detector technology | CMOS |
| Collection date | 2015-10-25 |
| Detector | RDI CMOS_8M |
| Wavelength(s) | 1.00001 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 171.980, 171.980, 99.900 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 60.943 - 2.500 |
| R-factor | 0.1944 |
| Rwork | 0.192 |
| R-free | 0.24270 |
| Starting model (for MR) | 5epc |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.125 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 60.943 | 2.410 |
| High resolution limit [Å] | 2.350 | 2.350 |
| Rmerge | 0.123 | 3.101 |
| Number of reflections | 71761 | |
| <I/σ(I)> | 16.5 | 0.8 |
| Completeness [%] | 99.8 | 99.2 |
| Redundancy | 14.1 | 12.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 1.5 M ammonium sulfate with 0.15 lithium sulfate and 0.1 CAPS buffer, pH 10.5 |
