5F8L
Enterovirus 71 Polymerase Elongation Complex (C3S1 Form)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-10-11 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97911 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 62.228, 76.709, 151.247 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.034 - 2.812 |
| R-factor | 0.2022 |
| Rwork | 0.200 |
| R-free | 0.23760 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ol6 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.163 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (phenix.refine: 1.9_1692) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 60.000 | 60.000 | 2.910 |
| High resolution limit [Å] | 2.810 | 6.050 | 2.810 |
| Rmerge | 0.102 | 0.045 | 0.500 |
| Rmeas | 0.111 | 0.049 | 0.543 |
| Rpim | 0.044 | 0.020 | 0.211 |
| Total number of observations | 116789 | ||
| Number of reflections | 18195 | ||
| <I/σ(I)> | 8.2 | ||
| Completeness [%] | 99.9 | 99.6 | 100 |
| Redundancy | 6.4 | 6.1 | 6.6 |
| CC(1/2) | 0.997 | 0.921 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 289 | 0.085M MES pH 6.5, 25.5 %(w/v) PEG 5000 monomethyl ether, 15%(v/v) Glycerol |
