5F8J
Enterovirus 71 Polymerase Elongation Complex (C1S4 Form)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-01-02 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97920 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 63.430, 76.572, 149.267 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.425 - 2.675 |
| R-factor | 0.1851 |
| Rwork | 0.183 |
| R-free | 0.22830 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ol6 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.167 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (phenix.refine: 1.9_1692) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 60.000 | 60.000 | 2.760 |
| High resolution limit [Å] | 2.660 | 5.730 | 2.660 |
| Rmerge | 0.073 | 0.030 | 0.500 |
| Rmeas | 0.081 | 0.034 | 0.559 |
| Rpim | 0.035 | 0.015 | 0.241 |
| Total number of observations | 99971 | ||
| Number of reflections | 20430 | ||
| <I/σ(I)> | 9.9 | ||
| Completeness [%] | 96.4 | 92.5 | 98.1 |
| Redundancy | 4.9 | 4.8 | 4.9 |
| CC(1/2) | 0.999 | 0.845 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 289 | 0.085M MES pH 6.5, 25.5%(w/v) PEG 5000 monomethyl ether, 15%(v/v) Glycerol |
