5F7V
ABC substrate-binding protein Lmo0181 from Listeria monocytogenes in complex with cycloalternan
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-F |
Synchrotron site | APS |
Beamline | 21-ID-F |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-04-27 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | .97872 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 49.034, 37.639, 90.426 |
Unit cell angles | 90.00, 93.21, 90.00 |
Refinement procedure
Resolution | 29.840 - 1.400 |
R-factor | 0.15293 |
Rwork | 0.151 |
R-free | 0.18099 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.010 |
RMSD bond angle | 1.853 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0107) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.420 |
High resolution limit [Å] | 1.400 | 1.400 |
Rmerge | 0.067 | 0.360 |
Number of reflections | 64488 | |
<I/σ(I)> | 15.9 | 3 |
Completeness [%] | 99.0 | 99.9 |
Redundancy | 3.1 | 3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 300 | 26 mg/mL protein in 0.05 M sodium chloride, 0.01 M Tris-HCl, pH 8.3, 2 mM cycloalternan against Classics II D4 (Qiagen, 0.1 M citric acid, pH 3.5, 25% w/v PEG3350) |