5F7T

TRIM5 B-box2 and coiled-coil chimera

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2015-07-08
Detector	RAYONIX MX300-HS
Wavelength(s)	1
Spacegroup name	P 1
Unit cell lengths	45.802, 52.329, 69.691
Unit cell angles	94.77, 105.54, 103.02

Refinement procedure

Resolution	35.052 - 2.292
R-factor	0.2254
Rwork	0.224
R-free	0.25640
RMSD bond length	0.004
RMSD bond angle	0.724
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.380
High resolution limit [Å]	2.292	4.950	2.300
Rmerge	0.068	0.052	0.164
Rmeas	0.096	0.073	0.232
Rpim	0.068	0.052	0.164
Total number of observations	48380
Number of reflections	25190
<I/σ(I)>	9.4
Completeness [%]	93.9	98.2	80
Redundancy	1.9	2	1.7
CC(1/2)		0.989	0.951

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	290	0.1M NaAcetate 4.6, 0.2M CaAcetate, 23% PEG 3350