5F7T
TRIM5 B-box2 and coiled-coil chimera
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-07-08 |
| Detector | RAYONIX MX300-HS |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 |
| Unit cell lengths | 45.802, 52.329, 69.691 |
| Unit cell angles | 94.77, 105.54, 103.02 |
Refinement procedure
| Resolution | 35.052 - 2.292 |
| R-factor | 0.2254 |
| Rwork | 0.224 |
| R-free | 0.25640 |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.724 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.380 |
| High resolution limit [Å] | 2.292 | 4.950 | 2.300 |
| Rmerge | 0.068 | 0.052 | 0.164 |
| Rmeas | 0.096 | 0.073 | 0.232 |
| Rpim | 0.068 | 0.052 | 0.164 |
| Total number of observations | 48380 | ||
| Number of reflections | 25190 | ||
| <I/σ(I)> | 9.4 | ||
| Completeness [%] | 93.9 | 98.2 | 80 |
| Redundancy | 1.9 | 2 | 1.7 |
| CC(1/2) | 0.989 | 0.951 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 290 | 0.1M NaAcetate 4.6, 0.2M CaAcetate, 23% PEG 3350 |
