5F7P
Rok Repressor Lmo0178 from Listeria monocytogenes
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-F |
| Synchrotron site | APS |
| Beamline | 21-ID-F |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-04-20 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.97872 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 68.970, 81.785, 141.089 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 70.757 - 2.840 |
| R-factor | 0.22622 |
| Rwork | 0.223 |
| R-free | 0.29257 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5f7r |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.694 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 70.757 | 2.900 |
| High resolution limit [Å] | 2.838 | 2.838 |
| Rmerge | 0.033 | 0.628 |
| Number of reflections | 19017 | |
| <I/σ(I)> | 27.1 | 2.3 |
| Completeness [%] | 97.7 | 98.7 |
| Redundancy | 4.8 | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 300 | 10 mg/mL proten in 10 mM Tris, pH 8.3, 100 mM sodium chloride against JCSG+ (Qiagen) A10 (0.2 M potassium formate, 20% PEG3350) |
