5F7L
Blood group antigen binding adhesin BabA of Helicobacter pylori strain 17875 in complex with Nanobody Nb-ER14
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 2 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-12-05 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9791 |
Spacegroup name | P 2 21 21 |
Unit cell lengths | 42.402, 133.715, 201.623 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.400 - 2.740 |
R-factor | 0.21494 |
Rwork | 0.212 |
R-free | 0.26486 |
Structure solution method | SAD |
RMSD bond length | 0.013 |
RMSD bond angle | 1.600 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | DM |
Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.400 | 2.880 |
High resolution limit [Å] | 2.740 | 2.740 |
Rmerge | 0.139 | 0.555 |
Number of reflections | 31163 | |
<I/σ(I)> | 7.9 | 2.7 |
Completeness [%] | 99.3 | 95.8 |
Redundancy | 5.8 | 5.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.2 M Lithium sulfate, 0.1 M Sodium acetate 4.5, 50 % w/v PEG 400 |