5F71

Human T-cell immunoglobulin and mucin domain protein 3 (hTIM-3)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2014-10-12
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.97479
Spacegroup name	P 21 21 21
Unit cell lengths	53.997, 61.957, 67.152
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.810 - 2.404
R-factor	0.2166
Rwork	0.215
R-free	0.25710
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2oyp
RMSD bond length	0.007
RMSD bond angle	1.252
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.7.3_928)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.490
High resolution limit [Å]	2.400	2.400
Rmerge	0.130	0.842
Number of reflections	9178
<I/σ(I)>	13.301
Completeness [%]	100.0	100
Redundancy	5.8	5.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	0.8M potassium sodium tartrate tetrahydrate, 0.1M sodium HEPES at pH 7.5