5F71
Human T-cell immunoglobulin and mucin domain protein 3 (hTIM-3)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-10-12 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.97479 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 53.997, 61.957, 67.152 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 34.810 - 2.404 |
R-factor | 0.2166 |
Rwork | 0.215 |
R-free | 0.25710 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2oyp |
RMSD bond length | 0.007 |
RMSD bond angle | 1.252 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX (1.7.3_928) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.490 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.130 | 0.842 |
Number of reflections | 9178 | |
<I/σ(I)> | 13.301 | |
Completeness [%] | 100.0 | 100 |
Redundancy | 5.8 | 5.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.8M potassium sodium tartrate tetrahydrate, 0.1M sodium HEPES at pH 7.5 |