5F6H
Crystal Structure of Tier 2 Neutralizing Antibody DH427 from a Rhesus Macaque
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-03-23 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97916 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 77.882, 153.786, 162.878 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.897 - 2.660 |
| R-factor | 0.232 |
| Rwork | 0.230 |
| R-free | 0.26190 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | separated Fv and Fc regions of I3.2 Fab from PDB Entry 4QHL |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.912 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.900 | 2.740 |
| High resolution limit [Å] | 2.660 | 2.690 |
| Rmerge | 0.171 | 0.922 |
| Number of reflections | 55490 | |
| <I/σ(I)> | 13.05 | 1.3 |
| Completeness [%] | 97.3 | 85.5 |
| Redundancy | 4.8 | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4 | 293 | 20% PEG 2K MME, 100 mM sodium citrate, pH 4.0, 100 mM NaCl |
