5F6F
S. aureus MepR G34R Mutant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-07-31 |
Detector | RAYONIX MX300-HS |
Wavelength(s) | 1 |
Spacegroup name | H 3 |
Unit cell lengths | 119.949, 119.949, 55.798 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 29.987 - 1.751 |
R-factor | 0.1899 |
Rwork | 0.187 |
R-free | 0.22600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5ffz |
RMSD bond length | 0.006 |
RMSD bond angle | 0.966 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.7.1_743) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.990 | 1.780 |
High resolution limit [Å] | 1.750 | 1.750 |
Rmerge | 0.080 | 0.825 |
Number of reflections | 29854 | |
<I/σ(I)> | 14.1 | |
Completeness [%] | 99.1 | 100 |
Redundancy | 5.5 | 5.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 298 | 25% (w/v) Peg 1500, 100 mM MIB buffer (Imidazole, Boric Acid, Sodium Malonate Dibasic Monohydrate) |