5F6D
Crystal structure of Ubc9 (K48A/K49A/E54A) complexed with Fragment 6
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-BM |
| Synchrotron site | APS |
| Beamline | 22-BM |
| Temperature [K] | 93 |
| Detector technology | CCD |
| Collection date | 2013-11-21 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 50.734, 35.452, 57.979 |
| Unit cell angles | 90.00, 111.63, 90.00 |
Refinement procedure
| Resolution | 26.949 - 1.553 |
| R-factor | 0.1725 |
| Rwork | 0.171 |
| R-free | 0.19620 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1u9b |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.122 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.580 |
| High resolution limit [Å] | 1.550 | 1.550 |
| Rmerge | 0.041 | 0.255 |
| Number of reflections | 26685 | |
| <I/σ(I)> | 34.9 | 3.1 |
| Completeness [%] | 95.3 | 70.9 |
| Redundancy | 3.6 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8.5 | 294 | 0.1M Tris pH 8.5, 8% (w/v) polyethylene glycol 8000 |
