5F5U
Crystal structure of the Snu23-Prp38-MFAP1(217-258) complex of Chaetomium thermophilum
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.2 |
| Synchrotron site | BESSY |
| Beamline | 14.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-03-13 |
| Detector | RAYONIX MX-225 |
| Wavelength(s) | 0.918 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 88.960, 95.260, 133.960 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.927 - 2.748 |
| R-factor | 0.2317 |
| Rwork | 0.229 |
| R-free | 0.27490 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4rz9 |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.836 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.930 | 2.820 |
| High resolution limit [Å] | 2.748 | 2.748 |
| Number of reflections | 30180 | |
| <I/σ(I)> | 11.1 | 1 |
| Completeness [%] | 99.5 | 98.9 |
| Redundancy | 5 | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 4.75 | 277.15 | 0.1M citric acid, pH 4.75, and 15% (w/v) PEG 6,000 |
