5F5T

Crystal structure of the Prp38-MFAP1 complex of Chaetomium thermophilum

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P14 (MX2)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-06-22
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.976
Spacegroup name	C 1 2 1
Unit cell lengths	195.550, 52.150, 104.600
Unit cell angles	90.00, 102.51, 90.00

Refinement procedure

Resolution	95.454 - 2.550
R-factor	0.1969
Rwork	0.196
R-free	0.22330
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4rz9
RMSD bond length	0.003
RMSD bond angle	0.814
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	95.454	2.620
High resolution limit [Å]	2.550	2.550
Number of reflections	33726
<I/σ(I)>	17.9	1.9
Completeness [%]	98.9	98.4
Redundancy	3.8	3.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	8.5	277.15	0.1 M Tris-HCl, pH 8.5, 0.2 M ammonium acetate, and 20 % (v/v) isopropanol