5F5S
Crystal structure of the Prp38-MFAP1 complex of Homo sapiens
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.2 |
| Synchrotron site | BESSY |
| Beamline | 14.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-03-01 |
| Detector | RAYONIX MX-225 |
| Wavelength(s) | 1.24 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 66.790, 66.790, 242.540 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 57.840 - 2.400 |
| R-factor | 0.2233 |
| Rwork | 0.221 |
| R-free | 0.26090 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4rz9 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.972 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 57.840 | 2.460 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Number of reflections | 13389 | |
| <I/σ(I)> | 24.6 | 1.9 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 18.5 | 19.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 4.4 | 277.15 | 0.1 M sodium acetate, pH 4.4 and 15% (v/v) 1,3-Butandiol |
