5F5R
TRAP1N-ADPNP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 93 |
| Detector technology | CCD |
| Collection date | 2013-02-17 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97926 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 92.570, 143.168, 103.011 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.410 - 1.850 |
| R-factor | 0.1771 |
| Rwork | 0.176 |
| R-free | 0.20300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2cg9 |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.656 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.410 | 1.880 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.055 | 0.527 |
| Number of reflections | 54569 | |
| <I/σ(I)> | 14.9 | 8.4 |
| Completeness [%] | 98.5 | 98 |
| Redundancy | 7.9 | 7.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 287 | 100 mM sodium citrate pH 5.6, 22% PEG4000, 5% isopropanol |
