5F56

Structure of RecJ complexed with DNA and SSB-ct

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	95
Detector technology	CCD
Collection date	2015-09-08
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9792
Spacegroup name	P 32 2 1
Unit cell lengths	102.220, 102.220, 166.120
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.054 - 2.300
R-factor	0.2262
Rwork	0.226
R-free	0.23890
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2zxp
RMSD bond length	0.012
RMSD bond angle	1.464
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((1.10_2155: 000))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000		2.350
High resolution limit [Å]	2.300	10.260	2.300
Rmerge	0.074	0.030	0.637
Rmeas	0.080	0.033	0.689
Total number of observations	328053
Number of reflections	45296	535	3130
<I/σ(I)>	17.03	41.58	2.95
Completeness [%]	99.4	90.8	94.1
Redundancy	7.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	298	MES, MnCl2, Li2SO4