5F54

Structure of RecJ complexed with dTMP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	95
Detector technology	CCD
Collection date	2015-05-19
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9792
Spacegroup name	P 32 2 1
Unit cell lengths	106.530, 106.530, 161.900
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.288 - 2.700
R-factor	0.2088
Rwork	0.206
R-free	0.25200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2zxp
RMSD bond length	0.012
RMSD bond angle	1.085
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((1.10_2155: 000))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000		2.770
High resolution limit [Å]	2.700	12.080	2.700
Rmerge	0.055	0.030	0.641
Rmeas	0.058	0.033	0.679
Total number of observations	264098
Number of reflections	29475	231	2141
<I/σ(I)>	27.02	44.32	4
Completeness [%]	98.9	58.5	99.5
Redundancy	8.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	298	MES, MnCl2, Li2SO4