5F4Y
Structure of the SD2 domain of Human Shroom2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-08-02 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1.1 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 90.887, 110.000, 118.862 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 14.932 - 3.293 |
| R-factor | 0.2531 |
| Rwork | 0.247 |
| R-free | 0.29890 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3thf |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.453 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((dev_2219)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.360 |
| High resolution limit [Å] | 3.293 | 3.293 |
| Rmerge | 0.080 | 0.524 |
| Number of reflections | 8522 | |
| <I/σ(I)> | 23.7 | 2.26 |
| Completeness [%] | 92.7 | 91.8 |
| Redundancy | 5.6 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 20% PEG 3350, 200 mM Magnesium Chloride, 100 mM Tris pH 8.5 |
