5F4U
HIV-1 gp120 complex with BNM-IV-197
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 180 |
| Detector technology | CCD |
| Collection date | 2015-10-12 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9792 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 67.439, 128.123, 193.599 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.400 - 3.100 |
| R-factor | 0.2208 |
| Rwork | 0.218 |
| R-free | 0.27990 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3tgr |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.175 |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 48.400 |
| High resolution limit [Å] | 3.100 |
| Rmerge | 0.133 |
| Number of reflections | 15645 |
| <I/σ(I)> | 9 |
| Completeness [%] | 99.9 |
| Redundancy | 5.88 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 290 | peg 1500,calcium chloride, imidazole |
