5F4T

Crystal structure of the human sperm Izumo1 residues 22-254

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	100
Detector technology	CCD
Collection date	2015-04-30
Detector	RAYONIX MX-300
Wavelength(s)	0.97949
Spacegroup name	H 3
Unit cell lengths	126.039, 126.039, 60.895
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	40.642 - 3.083
R-factor	0.2209
Rwork	0.219
R-free	0.24840
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5f4e
RMSD bond length	0.004
RMSD bond angle	0.938
Data reduction software	XDS
Data scaling software	Aimless (0.5.8)
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	63.020	40.640	3.300
High resolution limit [Å]	3.080	8.720	3.080
Rmerge	0.115	0.052	0.474
Rpim	0.080	0.036	0.343
Total number of observations	18861	831	2981
Number of reflections	6484
<I/σ(I)>	9	15.9	3.1
Completeness [%]	97.4	96	91.3
Redundancy	2.9	2.9	2.7
CC(1/2)	0.993	0.994	0.774

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.6	295	0.07 M sodium acetate (pH 4.6), 5.6% (w/v) PEG 4000 and 30% (v/v) glycerol