5F4R
HIV-1 gp120 complex with BNW-IV-147
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.2 |
Synchrotron site | ALS |
Beamline | 5.0.2 |
Temperature [K] | 180 |
Detector technology | CCD |
Collection date | 2015-09-06 |
Detector | MAR CCD 130 mm |
Wavelength(s) | 1.00004 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 67.679, 127.357, 192.064 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.016 - 2.800 |
R-factor | 0.259 |
Rwork | 0.257 |
R-free | 0.29650 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3tgr |
RMSD bond length | 0.004 |
RMSD bond angle | 1.036 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 48.016 |
High resolution limit [Å] | 2.800 |
Rmerge | 0.117 |
Number of reflections | 20923 |
<I/σ(I)> | 13.2 |
Completeness [%] | 99.8 |
Redundancy | 7.25 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | EVAPORATION | 290 | PEG 1500, calcium chloride, imidazole |