5F4Q

Crystal structure of the human egg surface protein Juno

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E SUPERBRIGHT
Temperature [K]	100
Detector technology	CCD
Collection date	2015-07-28
Detector	RIGAKU SATURN 92
Wavelength(s)	1.54
Spacegroup name	P 41
Unit cell lengths	73.150, 73.150, 163.100
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.680 - 1.800
R-factor	0.1985
Rwork	0.196
R-free	0.22660
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5f4e
RMSD bond length	0.008
RMSD bond angle	1.085
Data reduction software	XDS
Data scaling software	Aimless (0.5.8)
Phasing software	PHASER
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	43.680	43.680	1.840
High resolution limit [Å]	1.800	9.180	1.800
Rmerge	0.117	0.025	0.735
Rpim	0.044	0.010	0.284
Total number of observations	640808	4586	34618
Number of reflections	79079
<I/σ(I)>	17.5	62.6	3
Completeness [%]	100.0	99.2	99.8
Redundancy	8.1	7.4	7.6
CC(1/2)	0.999	1.000	0.898

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	295	0.02 M magnesium chloride, 0.1 M HEPES sodium salt [pH 7.5], 22% (w/v) Polyacrylic acid 5100