5F4P
HIV-1 gp120 complex with BNM-III-170
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 180 |
Detector technology | PIXEL |
Collection date | 2015-04-17 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 0.9792 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 67.378, 127.517, 192.945 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.236 - 2.600 |
R-factor | 0.2754 |
Rwork | 0.274 |
R-free | 0.31050 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3tgr |
RMSD bond length | 0.006 |
RMSD bond angle | 1.064 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 48.236 |
High resolution limit [Å] | 2.500 |
Rmerge | 0.092 |
Number of reflections | 26041 |
<I/σ(I)> | 12.1 |
Completeness [%] | 99.2 |
Redundancy | 6.29 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | EVAPORATION | 290 | PEG 1500, calcium magnesium chloride, imidazole |