5F4M

Protruding domain of GII.17 norovirus Kawasaki323

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2015-08-12
Detector	MAR scanner 345 mm plate
Wavelength(s)	1.54179
Spacegroup name	P 43 21 2
Unit cell lengths	121.510, 121.510, 156.600
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.606 - 2.270
R-factor	0.1608
Rwork	0.159
R-free	0.18680
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	homology model based on 4OOS
RMSD bond length	0.007
RMSD bond angle	1.081
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER (2.5.6)
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	19.610		2.300
High resolution limit [Å]	2.240	10.030	2.240
Rmerge	0.156	0.054	0.879
Rmeas	0.176	0.061	1.001
Total number of observations	495136
Number of reflections	106195	1060	6893
<I/σ(I)>	7.76	21.37	1.7
Completeness [%]	98.8	86.5	86.4
Redundancy	8.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	291	Magnesium Chloride, PEG8000, Tris-HCl