5F4H

Archael RuvB-like Holiday junction helicase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-05-06
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97
Spacegroup name	P 1 21 1
Unit cell lengths	101.720, 148.980, 122.130
Unit cell angles	90.00, 104.07, 90.00

Refinement procedure

Resolution	39.190 - 2.699
R-factor	0.2293
Rwork	0.227
R-free	0.26500
Structure solution method	MAD
RMSD bond length	0.018
RMSD bond angle	1.931
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.190	2.769
High resolution limit [Å]	2.699	2.699
Rmerge	0.145	2.453
Number of reflections	89886
<I/σ(I)>	16.1	4.3
Completeness [%]	97.6	92.67
Redundancy	7	6.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5	298	3:2 ratio of protein to mother liquor (0.1 M sodium malonate pH 5.0, 12% PEG 3350, 3% glycerol)