5F4H
Archael RuvB-like Holiday junction helicase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL12-2 |
Synchrotron site | SSRL |
Beamline | BL12-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-05-06 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 101.720, 148.980, 122.130 |
Unit cell angles | 90.00, 104.07, 90.00 |
Refinement procedure
Resolution | 39.190 - 2.699 |
R-factor | 0.2293 |
Rwork | 0.227 |
R-free | 0.26500 |
Structure solution method | MAD |
RMSD bond length | 0.018 |
RMSD bond angle | 1.931 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 39.190 | 2.769 |
High resolution limit [Å] | 2.699 | 2.699 |
Rmerge | 0.145 | 2.453 |
Number of reflections | 89886 | |
<I/σ(I)> | 16.1 | 4.3 |
Completeness [%] | 97.6 | 92.67 |
Redundancy | 7 | 6.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5 | 298 | 3:2 ratio of protein to mother liquor (0.1 M sodium malonate pH 5.0, 12% PEG 3350, 3% glycerol) |