5F4C
Crystal Structure of Ribonuclease Inhibitor Barstar from Salmonella Typhimurium
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-12-01 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 29.362, 52.790, 58.812 |
| Unit cell angles | 90.00, 93.35, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.400 |
| R-factor | 0.14321 |
| Rwork | 0.141 |
| R-free | 0.17669 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2cx6 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.455 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.420 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Number of reflections | 34560 | |
| <I/σ(I)> | 28.59 | 1.87 |
| Completeness [%] | 97.8 | 80 |
| Redundancy | 4.7 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 289 | 2.4M Sodium malonate, pH 7.0 |
