5F42
Activity and Crystal Structure of Francisella novicida UDP-N-Acetylglucosamine Acyltransferase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2009-03-17 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 105.380, 105.380, 285.790 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 18.950 - 2.060 |
| R-factor | 0.21225 |
| Rwork | 0.210 |
| R-free | 0.25968 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1lxa |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.112 |
| Data scaling software | XDS |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.490 | 2.110 |
| High resolution limit [Å] | 2.060 | 2.060 |
| Number of reflections | 35991 | |
| <I/σ(I)> | 8.02 | |
| Completeness [%] | 100.0 | |
| Redundancy | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.2M Lithium sulfate, 0.1M Tris HCl pH 8.5, 30% PEG 4000 |
