5F42
Activity and Crystal Structure of Francisella novicida UDP-N-Acetylglucosamine Acyltransferase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2009-03-17 |
Detector | RIGAKU RAXIS IV++ |
Wavelength(s) | 1.5418 |
Spacegroup name | H 3 2 |
Unit cell lengths | 105.380, 105.380, 285.790 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 18.950 - 2.060 |
R-factor | 0.21225 |
Rwork | 0.210 |
R-free | 0.25968 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1lxa |
RMSD bond length | 0.008 |
RMSD bond angle | 1.112 |
Data scaling software | XDS |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 19.490 | 2.110 |
High resolution limit [Å] | 2.060 | 2.060 |
Number of reflections | 35991 | |
<I/σ(I)> | 8.02 | |
Completeness [%] | 100.0 | |
Redundancy | 4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.2M Lithium sulfate, 0.1M Tris HCl pH 8.5, 30% PEG 4000 |