5F3P

Crystal structure of a noncanonical Dicer protein from Entamoeba histolytica

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2014-03-18
Detector	ADSC QUANTUM 315
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	46.092, 89.956, 100.358
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.730 - 1.904
R-factor	0.209
Rwork	0.207
R-free	0.23980
RMSD bond length	0.008
RMSD bond angle	0.804
Refinement software	PHENIX ((1.10_2155))

Data quality characteristics

	Overall
Low resolution limit [Å]	30.000
High resolution limit [Å]	1.800
Number of reflections	31630
<I/σ(I)>	26.8
Completeness [%]	94.8
Redundancy	6.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		289	1.6 M (NH4)2SO4, 0.01 M MgCl2, 0.05 M Tris-HCl pH 7.5