5F3O

Crystal structure of EhRNaseIII229 from Entamoeba histolytica complexed with Mn2+

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2014-04-24
Detector	ADSC QUANTUM 315
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	46.329, 89.729, 100.196
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.864 - 2.050
R-factor	0.1992
Rwork	0.197
R-free	0.24010
RMSD bond length	0.003
RMSD bond angle	0.574
Refinement software	PHENIX ((1.10_2155))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.050
Number of reflections	25785
<I/σ(I)>	16.4
Completeness [%]	95.3
Redundancy	5.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	289	1.6M (NH4)2SO4, 0.01M MgCl2, 0.05M Tris-HCl pH 7.5