5F3J
Crystal structure of DBP in complex with inhibitory monoclonal antibody 2D10
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 4.2.2 |
| Synchrotron site | ALS |
| Beamline | 4.2.2 |
| Temperature [K] | 93 |
| Detector technology | CMOS |
| Collection date | 2015-02-25 |
| Detector | RDI CMOS_8M |
| Wavelength(s) | 1.00482 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 84.940, 63.940, 108.880 |
| Unit cell angles | 90.00, 100.09, 90.00 |
Refinement procedure
| Resolution | 19.944 - 4.001 |
| R-factor | 0.2686 |
| Rwork | 0.267 |
| R-free | 0.29970 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4nuv |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.678 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.10_2155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 4.100 |
| High resolution limit [Å] | 4.000 | 4.000 |
| Rmerge | 0.116 | 0.275 |
| Number of reflections | 9271 | |
| <I/σ(I)> | 6.74 | 3.29 |
| Completeness [%] | 92.9 | 91 |
| Redundancy | 2.73 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 290 | 1% w/v tryptone, 50 mM HEPES sodium salt pH 7.0, 12% w/v PEG 3,350 |
