5F31
Crystal structure of membrane associated PatA from Mycobacterium smegmatis in complex with palmitate - P 42 21 2 space group
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-12-19 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.99996 |
| Spacegroup name | P 42 21 2 |
| Unit cell lengths | 80.302, 80.302, 113.766 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.187 - 2.430 |
| R-factor | 0.2266 |
| Rwork | 0.224 |
| R-free | 0.27930 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.755 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.190 | |
| High resolution limit [Å] | 2.430 | 2.430 |
| Rmerge | 0.122 | 0.669 |
| Number of reflections | 14652 | |
| <I/σ(I)> | 4.6 | 1.1 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 10.5 | 10.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 291 | 100 mM Tris-HCl pH 8.5 and 20% ethanol |
