5F2D
Crystal structure of Staphylococcal nuclease variant Delta+PHS I92N/V99T at cryogenic temperature
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SEALED TUBE |
| Source details | OTHER |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2014-03-13 |
| Detector | APEX II CCD |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 31.118, 60.503, 38.184 |
| Unit cell angles | 90.00, 93.28, 90.00 |
Refinement procedure
| Resolution | 38.120 - 1.700 |
| R-factor | 0.1917 |
| Rwork | 0.190 |
| R-free | 0.22160 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3bdc |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.836 |
| Data reduction software | APEX 2 |
| Data scaling software | APEX 2 |
| Phasing software | PHASER (2.5.2) |
| Refinement software | REFMAC (5.8.0131) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.720 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.014 | 0.112 |
| Total number of observations | 393870 | |
| Number of reflections | 15639 | |
| <I/σ(I)> | 48.29 | 6.73 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 25.14 | 8.74 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9 | 277 | 20% MPD, 25 mM potassium phosphate, calcium chloride, pdTp |
