5F26
Crystal structures of Pribnow box consensus promoter sequence (P63)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-X |
| Temperature [K] | 150 |
| Detector technology | PIXEL |
| Collection date | 2015-08-11 |
| Detector | DECTRIS PILATUS 200K |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 63 |
| Unit cell lengths | 49.232, 49.232, 67.679 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 36.074 - 3.000 |
| R-factor | 0.289 |
| Rwork | 0.288 |
| R-free | 0.29830 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 463d |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.720 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.640 | 3.000 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.027 | 0.157 |
| Number of reflections | 2101 | |
| <I/σ(I)> | 16.94 | 1.78 |
| Completeness [%] | 99.6 | 100 |
| Redundancy | 1.93 | 1.97 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | DNA, sodium cacodylate, barium chloride, potassium chloride, spermine tetrahydrochloride, 2-methyl-2,4-pentanediol |
