5F22

C-terminal domain of SARS-CoV nsp8 complex with nsp7

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-05-04
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.977
Spacegroup name	C 2 2 21
Unit cell lengths	50.862, 71.439, 115.383
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.719 - 2.155
R-factor	0.216
Rwork	0.212
R-free	0.25230
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.020
RMSD bond angle	1.302
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.7_650)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	1.600
Number of reflections	11223
<I/σ(I)>	59.3
Completeness [%]	100.0
Redundancy	18.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289	0.2M magnesium acetate, PEG 3350