5F1S

Crystal structure of the teleost fish polymeric Ig receptor (pIgR) ectodomain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2012-07-19
Detector	PSI PILATUS 6M
Wavelength(s)	0.9537
Spacegroup name	P 43 21 2
Unit cell lengths	54.938, 54.938, 187.257
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.037 - 1.749
R-factor	0.1881
Rwork	0.185
R-free	0.21480
Structure solution method	SAD
RMSD bond length	0.014
RMSD bond angle	1.216
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	SHELXDE
Refinement software	PHENIX (1.10_2155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.040	1.810
High resolution limit [Å]	1.749	1.749
Rmerge	0.054	1.050
Number of reflections	30034
<I/σ(I)>	18.17	1.9
Completeness [%]	99.8	98.95
Redundancy	7.7	8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	289	75mM MES monohydrate pH 6.5, 9% w/v PEG 20,000, 25mM phenol