5F1P
Crystal Structure of Dehydrogenase from Streptomyces platensis
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-08-01 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97921 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 77.368, 77.953, 81.575 |
Unit cell angles | 90.00, 105.47, 90.00 |
Refinement procedure
Resolution | 40.921 - 2.099 |
R-factor | 0.1921 |
Rwork | 0.190 |
R-free | 0.24170 |
Structure solution method | SAD |
RMSD bond length | 0.009 |
RMSD bond angle | 1.096 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | HKL-3000 |
Refinement software | PHENIX (dev_1839) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.140 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.707 | |
Number of reflections | 27188 | |
<I/σ(I)> | 15.4 | 1.44 |
Completeness [%] | 99.3 | 89.5 |
Redundancy | 3.7 | 2.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 289 | 0.2 M sodium formate, 20 %(w/v) PEG 3350 |