5F1P
Crystal Structure of Dehydrogenase from Streptomyces platensis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-08-01 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97921 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 77.368, 77.953, 81.575 |
| Unit cell angles | 90.00, 105.47, 90.00 |
Refinement procedure
| Resolution | 40.921 - 2.099 |
| R-factor | 0.1921 |
| Rwork | 0.190 |
| R-free | 0.24170 |
| Structure solution method | SAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.096 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | PHENIX (dev_1839) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.140 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.707 | |
| Number of reflections | 27188 | |
| <I/σ(I)> | 15.4 | 1.44 |
| Completeness [%] | 99.3 | 89.5 |
| Redundancy | 3.7 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 289 | 0.2 M sodium formate, 20 %(w/v) PEG 3350 |
