5F1N

MHC complexed to 11mer peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2015-02-14
Detector	BIODIFF
Wavelength(s)	0.97915
Spacegroup name	P 1 21 1
Unit cell lengths	49.312, 160.492, 64.481
Unit cell angles	90.00, 104.08, 90.00

Refinement procedure

Resolution	31.454 - 2.000
R-factor	0.1855
Rwork	0.184
R-free	0.22050
RMSD bond length	0.007
RMSD bond angle	0.996
Data reduction software	CNS
Data scaling software	CNS
Phasing software	PHASES
Refinement software	PHENIX (1.8.4_1496)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.000
Number of reflections	64847
<I/σ(I)>	15.997
Completeness [%]	99.4
Redundancy	4.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	291	0.2 M sodium chloride, 0.1 M Bis-Tris (pH 5.5), 25% PEG 3,350 (w/v)