5F1F
Crystal structure of CMY-10 adenylylated by acetyl-AMP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 5C (4A) |
Synchrotron site | PAL/PLS |
Beamline | 5C (4A) |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-03-16 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.00000 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 49.874, 59.342, 111.841 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 38.180 - 1.548 |
R-factor | 0.1673 |
Rwork | 0.166 |
R-free | 0.19650 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1zkj |
RMSD bond length | 0.013 |
RMSD bond angle | 1.453 |
Data scaling software | HKL-2000 |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 1.548 |
Number of reflections | 44969 |
<I/σ(I)> | 62 |
Completeness [%] | 96.4 |
Redundancy | 6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | MICROBATCH | 6.5 | 295 | Cadmium chloride, Na-Cacodylate, PEG 400 |