5F1E

Apo protein of Sandercyanin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-06-24
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.987
Spacegroup name	P 63 2 2
Unit cell lengths	158.764, 158.764, 84.795
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	53.400 - 2.700
R-factor	0.199
Rwork	0.197
R-free	0.24810
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5f6z
RMSD bond length	0.009
RMSD bond angle	1.088
Data reduction software	iMOSFLM
Data scaling software	d*TREK
Phasing software	d*TREK
Refinement software	PHENIX (1.8.1_1168)

Data quality characteristics

	Overall
Low resolution limit [Å]	53.400
High resolution limit [Å]	2.700
Number of reflections	17824
<I/σ(I)>	31.48
Completeness [%]	100.0
Redundancy	36.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	0.1M HEPES, 10% PEG 8000, 8% v/v Ethylene glycol