5F16

CTA-modified hen egg-white lysozyme

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 4.2.2
Synchrotron site	ALS
Beamline	4.2.2
Temperature [K]	100
Detector technology	CMOS
Collection date	2014-08-22
Detector	RDI CMOS_8M
Wavelength(s)	1.000
Spacegroup name	P 43 21 2
Unit cell lengths	78.111, 78.111, 37.610
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	55.233 - 1.200
R-factor	0.211
Rwork	0.209
R-free	0.24840
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5f14
RMSD bond length	0.006
RMSD bond angle	0.989
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	55.233	1.260
High resolution limit [Å]	1.200	1.200
Rmerge	0.094	1.337
Number of reflections	36732
<I/σ(I)>	14.9	1.6
Completeness [%]	99.3	99.7
Redundancy	12.9	11.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	298	2M sodium chloride, 12% (w/v) PEG 6000