5F11
Crystal structure of Fructokinase from Vibrio cholerae O395 in fructose bound form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2013-07-16 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 117.533, 64.897, 41.953 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.667 - 2.300 |
| R-factor | 0.1905 |
| Rwork | 0.187 |
| R-free | 0.25830 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1tz6 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.094 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8_1069) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.350 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.100 | 0.200 |
| Number of reflections | 15710 | |
| <I/σ(I)> | 7.1 | 1.7 |
| Completeness [%] | 94.0 | 96.6 |
| Redundancy | 3.3 | 3.13 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | 5% PEG 6000, 0.1M MES, pH 6.0, 7 days |
